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  3. LacZ activity was found in discrete locations in embryos from to days post coitum (dpc), indicating that Smad8 transcription is developmentally regulated (Fig. 2). At and dpc, LacZ expression was detected in the cardiac outflow tract (OFT), in both the myocardium and cushion mesenchyme (Fig. 2 A–D).
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  5. Aug 01,  · Generation of the Smad8 ex4,5 and Smad8 lacZ alleles. To study the Smad8 function, we generated the Smad8 ex4,5 and Smad8 lacZ alleles using gene targeting in embryonic stem cells (Materials and Methods and Fig. 1).In the Smad8 flox allele, exons 4 and 5 were flanked by two LoxP sites with a Frt flanked Pgk Neomycin resistance cassette located 3′ of exon 5 (Fig. 1 A–E).
  6. Nov 26,  · HPGD knockout mice have normal appearing pregnancies at dpc and but lose their pregnancy by d.p.c (A) Dissected uterus at d.p.c .
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  8. Introduction. Prostaglandins (PGs) have critical signaling functions in a variety of processes including reproduction, blood pressure homeostasis, sexual dimorphism and inflammation 1 – cielimnahoubozycimortlogtiodapho.coinfo 2 and PGF 2α, have critical signaling functions in determining the termination of labor at term and prior to term in humans and mice 6 – 8. hydroxyprostaglandin dehydrogenase (HPGD) is the.
  9. The selection cassette Neo-Tk is represented as red and green boxes and the DTA-cassette as a blue box. Cre-mediated recombination (dashed lines) was used to obtain Separase floxed or Δ alleles. (B) Southern blot to confirm germ-line transmission of Separase flox and Δ alleles with EcoRV-digested DNA and the internal probe cielimnahoubozycimortlogtiodapho.coinfo by:

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